E-Z Freezin CryoMax LE Canine Freezing Extender, 3ml (pack of 12)

Cryomax™ LE - a superior generation of canine semen freezing extenders
Best in the industry providing superior results over other commercially available extenders.

E-Z Freezin® CryoMax™ extenders are packaged in 3 ml hermetically sealed packets and sold in 12-packs containing a total volume of 36 ml. All ARS extenders are evaluated by the Colorado State University Animal Reproduction and Biotechnology Laboratory to ensure a superior quality product.

E-Z Freezin® Cryomax™ Canine Semen Freezing Extenders are the result of the latest collaboration between Colorado State University and Animal Reproduction Systems, Inc. 

All shipments of E-Z Freezin® CryoMax™ Extenders include certification of production using eggs that are produced by a flock that is certified Specific Pathogen Free. The flock is certified free of the following pathogens: Avian Adenovirus Group I, Avian Adenovirus Group II (HEV), Avian Adenovirus Group III (EDS), Avian Encephalomyelitis, Avian Influenza (Type A), Avian Reovirus, Fowl Pox, Infectious Bronchitis-Ark., Infectious Bronchitis-Conn., Infectious Bronchitis-JMK, Infectious Bronchitis-Mass., Infectious Bursal Disease, Infectious Laryngotracheitis, Lymphoid Leukosis (A, B), Lymphoid Leukosis Viruses, Marek's Disease (Serotypes 1,2,3), Mycoplasma gallisepticum, Mycoplasma synoviae, Newcastle Disease, Reticuloendotheliosis Virus, Salmonella enterica seruvari, Pullorum and Gallinarum, and Salmonella.

Research at the Colorado State University has determined that thawing during shipment and subsequent refreezing does not adversely affect this product and have decided to have their E-Z Freezin® Cryomax™ Extenders shipped using more economical services during the cooler months of the year.

 Each E-Z Freezin® lot is evaluated by the CSU to ensure a superior product.

A series of studies were performed at the Equine Reproduction Laboratory, Colorado State University to compare commercial extenders for cryopreservation of stallion semen. Three freezing extenders contained a combination of glycerol and an amide as cryoprotectants (BotuCrio®, CryoMax™ LE and CryoMax™ MFR5), while two freezing extenders utilized glycerol as the only cryoprotectant (LE, MFR5).

Study 1. Semen was collected from a total of 12 different stallions. Aliquots of each ejaculate were diluted in one of five different extenders, loaded into 0.5 ml straws, cooled to 5 to 8° C over a 30 to 60 minute period and subsequently cryopreserved. One representative straw from each extender for every stallion was thawed in a 37° C water bath for 30 seconds. The thawed sperm was allowed to warm for 10 minutes and then evaluated for total and progressive motility using a computer assisted semen analysis (CASA) system. The highest average post-thaw motility was noted for the CryoMax™ LE extender (Table 1).

Table 1. Post-thaw motility for spermatozoa from 12 stallions cryopreserved in each of 5 commercial freezing extenders. Data are presented as the mean ? SEM.

ab Values within a column with different superscripts are significantly different (p<0.05) [ANOVA] xy Values are statistically different (p=0.032) [paired T-test]

Study 2. Semen was collected from 30 different stallions. Aliquots of each ejaculate were diluted in one of four commercial extenders, loaded into 0.5 ml straws, cooled to 5 to 8° C and cryopreserved. One straw from each extender for every stallion was thawed in a 37° C water bath for 30 seconds. The thawed sperm was allowed to warm for 10 minutes and then evaluated for total and progressive motility using a CASA system. Again, the highest average post-thaw motility was noted for the CryoMax™ LE and CryoMax™ MFR5 extenders (Table 2).

Table 2. Post-thaw motility for spermatozoa from 30 stallions cryopreserved in each of 4 commercial freezing extenders (mean ? SEM).

Summary. Freezing extenders containing a combination of glycerol and amide cryoprotectants yielded better post-thaw sperm motility than extenders containing glycerol alone. CryoMax™ LE extender yielded a higher post-thaw total motility when directly compared to BotuCrio® extender.